Advances in molecular technologies over recent years have a profound impact on diagnostic pathology. Molecular biology techniques utilize DNA, RNA, and enzymes that interact with nucleic acids to understand biology at a molecular level. Information derived from genomics and proteomics have permitted improved insights into a range of disorders and have led to improvements in the understanding and classification of many diseases. These advances will provide a complementary approach to traditional methods of histopathological analysis in the future. Molecular analysis will significantly impact three major areas: specific diagnosis, prognosis determination and prediction of response to therapy. The pathologist will continue to play a fundamental, role in diagnosis and will likely be in a pivotal position to guide the implementation and interpretation of these tests as they move from the research laboratory into diagnostic pathology.
In general any disease diagnosed clinically should be confirmed by a pathologist. Histopathology is considered as the gold standard technique for diagnosis, but the establishment of molecular structure of a cell had lead to a revolution in the field of diagnostic pathology. By the time the histopathology satisfies the criteria of a particular disease, the biochemical and genetic alterations would have progressed far in advance that requires much of attention. The molecular insight of human genome had lead to better understanding of disease course and appropriate treatment modalities thereby reducing the morbidity and mortality. The advanced molecular techniques have become an integral part of day to day investigative procedures and it is likely that in future, these methods will increasingly become a standard practice in routine diagnosis and management of the patients.
In recent years cellular pathology has become more closely involved in the direct management of patients with the introduction of molecular technologies and targeted therapies. Advanced Techniques in Diagnostic Cellular Pathology introduces students and professionals to these concepts and the key technologies that are influencing clinical practice today. Each chapter is carefully structured to introduce the very latest techniques and describe their clinical purpose, principle, method and application in cellular pathology. The advantages of various methods for preparing, observing and demonstrating cells and tissues employed to assist in diagnosis are explored, in addition to the use of quantitative methods in the detection and diagnosis of disease. Supplementary web-based material including annotated virtual microscope slides is available with the book. This is provided courtesy of i-Path Diagnostics Ltd and can be accessed online from their website www.pathxl.com Describes the very latest, emerging and established molecular aspects of diagnostic pathology. A clear, focused approach with each chapter containing a summary, a review of basic principles and clinical applications. Includes web-based annotated virtual microscope slides. Contributions from experienced practitioners contain numerous real-world examples illustrating the use of different diagnostic techniques, and their clinical relevance Written by a team of experienced practitioners this book will prove invaluable both to postgraduate biomedical science students who are training to be cellular pathologists and to professionals working in diagnostic and research laboratories as part of their continuing professional development.
The analysis of DNA, RNA and proteins obtained from diagnostic specimens, is currently revolutionizing the practice of surgical pathology and heralds a new era of diagnostic and prognostic tests that will greatly influence our day to day clinical decision making. The diagnosis of cancer and many other diseases is fundamentally based on the microscopic study of cells and tissues. Nevertheless, the era of the pathologist relying entirely on the examination of tissue sections stained by histochemical methods is gradually being replaced by a time when advanced immunologic and molecular techniques augment the process by which complicated infectious, inflammatory, metabolic and neoplastic diseases are diagnosed and classified. The pathologists will continue to play a fundamental role in diagnosis and will likely be in a pivotal position to guide the implementation and interpretation of these tests as they move from the research laboratory into diagnostic pathology. As we move further into a new era, the usefulness and ease of implementation of molecular test
This books presents the various diagnostic aids used in the early detection of precancerous and cancerous lesions.This book is of interest to students and clinicians from the field of Oral medicine ,oral pathology and oral surgery.A classification for the diagnostic aids has been written.This book will highlight on the various cancerous and precancerous lesions of the oral cavity.A with a discussion about the etiology,clinical features and pictorial description.The screening tests are discussed in detail.The screening tests include vital staining methods,liquid based cytology,light based detection systems and optical spectroscopy.Imaging modalities for detection of Oral cancer have been discussed.Molecular carcinogenesis and molecular markers have been elaborated in detail.The use of saliva as a diagnostic tool for Salivary biomarkers is also highlighted.This book promises its readers useful information for clinical and academic purpose.
Most textbooks on neurodegenerative disorders have used a classification scheme based upon either clinical syndromes or anatomical distribution of the pathology. In contrast, this book looks to the future and uses a classification based upon molecular mechanisms, rather than clinical or anatomical boundaries. Major advances in molecular genetics and the application of biochemical and immunocytochemical techniques to neurodegenerative disorders have generated this new approach. Throughout most of the current volume, diseases are clustered according to the proteins that accumulate within cells (e.g. tau, α-synuclein and TDP-43) and in the extracellular compartments (e.g. β-amyloid and prion proteins) or according to a shared pathogenetic mechanism, such as trinucleotide repeats, that are a feature of specific genetic disorders. Chapters throughout the book conform to a standard lay-out for ease of access by the reader and are written by a panel of International Experts Since the first edition of this book, major advances have been made in the discovery of common molecular mechanisms between many neurodegenerative diseases most notably in the frontotemporal lobar degenerations (FTLD) and motor neuron disease or amyotrophic lateral sclerosis. This book will be essential reading for clinicians, neuropathologists and basic neuroscientists who require the firm up-to-date knowledge of mechanisms, diagnostic pathology and genetics of Neurodegenerative diseases that is required for progress in therapy and management.
Telomerase activity, hTERT and Survivin these markers with combined urinary cytology may give more sensitive diagnostic clues in bladder malignancies and by using this approach diagnosis of low grade tumor are also possible which was not detectable by earlier nioninvasive methods. Diagnostic future of telomerase is very positive because today it is confirmed that near about all tumor cells express telomerase, so telomerase can help in early diagnosis and this way patients can get better treatment.
Now in its second edition, the Oxford Handbook of Clinical Pathology is the concise and rapidly accessible guide to general pathologic principles, and the pathology behind common and important conditions across all the major medical and surgical specialties. Fully updated and now including a brand new chapter on head and neck pathology, this book continues to be the definitive resource for medical students and doctors with an interest in this important specialty. Authored by leading experts, The Oxford Handbook of Clinical Pathology uses the best-selling Oxford Handbook format to cover macroscopic and microscopic features alongside immunohistochemical and molecular pathology in a succinct and practical style. This edition is fully illustrated and now includes over new 50 full colour images and staging information for major malignancies to aid the reader and to explain the pathology of common conditions.
Fish Pathology is the definitive, classic and essential book on the subject, providing in-depth coverage across all major aspects of fish pathology. This new, fully updated and expanded fourth edition builds upon the success of the previous editions which have made Fish Pathology the best known and most respected book in the field, worldwide.Commencing with a chapter covering the aquatic environment, the book provides comprehensive details of the anatomy and physiology of teleosts, pathophysiology and sytematic physiology, immunology, neoplasia, virology, parasitology, bacteriology, mycology, nutritional pathology and other non-infectious diseases. A final chapter provides extremely useful details of the most widely-used and trusted laboratory methods in the area. Much new infomation is included in this new edition, including enhanced coverage of any diseases which have become commercially significant since publication of the previous edition Beautifully illustrated in full colour throughout with many exceptional photographs, Fish Pathology, Fourth Edition, is an essential purchase for fish pathologists, fish veterinarians, biologists, microbiologists and immunologists, including all those working in diagnostic services worldwide. Personnel working in fish farming and fisheries will also find much of great use and interest within the book's covers. All libraries in universities and research establishments where biological and veterinary sciences are studied and taught should have copies of this landmark publication on their shelves.
This book represents a comprehensive and detailed display of some of the recent diagnostic methods used in early diagnosis of sepsis especially in neonates. It also comprises a statistical comparison between the diagnostic performance of these methods versus other conventional methods used in routine clinical practice. This is accomplished through an observational case-control study. We tried to reinforce, and sometimes weaken, our findings with the results of other relevant studies in order to offer a rather fair judgmental view about the value of different methods used.
Diagnostic pathology is indeed so vast, that a binocular vision is often needed to unravel the mystery of the many concurrent interactions occurring at different sites of the lesional tissue. Immunohistochemistry provides the pathologist with the right perspective in order to identify the complexity of a disease process. This library work covers selected topics and references, so that I and the readers are not overwhelmed by the otherwise exhaustive technical aspects of the many observed phenomena and the subsequent diverse interpretations of the same. I hope professionals and students in the field of oral pathology will find this book a relevant source of the procedures and the applications of the very intriguing aspect of diagnostic pathology
This book describes different laboratory methods for diagnosis of infectious diseases. There specific highlight on molecular techniques. Different molecular biological methods are described. There is also fine description of different laboratory techniques used in diagnosis of infectious diseases. There are different colour figures to make it easy to show the technology. The book is supplied with tables to describe different procedures. There is a description for use of molecular techniques in diagnosis of blood stream infections.
Acinetobacter baumannii has been recently considered an important nosocomial pathogen responsible of worldwide, intensively documented, outbreaks. This increases the need for effective epidemiological typing methods. Throughout the years, many typing methods for A. baumannii epidemiological studies have been proposed from phenotypic to molecular methods. Currently, the use of phenotypic typing methods have declined considerably and been progressively replaced by molecular methods. Many molecular typing methods have been described and of those, Pulsed Field Gel Electrophoresis remains the gold standard. Data about epidemic clones of Acinetobacter baumannii that caused outbreaks in developing counteries are lacking and need to be documented by continous research projects. This is particulary important because global spread of multidrug and pandrug resistant strains is a common problem facing us.
In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
The past 25 years have seen immense development of use of physical and statistical methods in medicine and molecular biology. This trend has not deteriorated, but rather tends to enforce itself in an ever mightier form. Currently, it is not possible to imagine a scientific research in medicine and molecular biology without vast employment of very sophisticated devices utilizing a wide array of physical and statistical methods of data processing. On the other hand, there is an endless scope of modern, mostly computerized, mathematical methods. Actually, there has been as though unnoticed rise of a huge „vacuum of unapplied and unrealized mathematical methods for solving problems in medicine and molecular biology, but also in other areas“. Many problems in medicine and molecular biology are solvable through crucially major contribution of the methods outlined. A lot of partial algorithmic problems will have to be yet analyzed in detail so that they become fully reliable. Upon their resolution, it shall be possible (in a historically short period) to construct a software which would enable to solve all of the indicated problems at user level.