In recent years cellular pathology has become more closely involved in the direct management of patients with the introduction of molecular technologies and targeted therapies. Advanced Techniques in Diagnostic Cellular Pathology introduces students and professionals to these concepts and the key technologies that are influencing clinical practice today. Each chapter is carefully structured to introduce the very latest techniques and describe their clinical purpose, principle, method and application in cellular pathology. The advantages of various methods for preparing, observing and demonstrating cells and tissues employed to assist in diagnosis are explored, in addition to the use of quantitative methods in the detection and diagnosis of disease. Supplementary web-based material including annotated virtual microscope slides is available with the book. This is provided courtesy of i-Path Diagnostics Ltd and can be accessed online from their website www.pathxl.com Describes the very latest, emerging and established molecular aspects of diagnostic pathology. A clear, focused approach with each chapter containing a summary, a review of basic principles and clinical applications. Includes web-based annotated virtual microscope slides. Contributions from experienced practitioners contain numerous real-world examples illustrating the use of different diagnostic techniques, and their clinical relevance Written by a team of experienced practitioners this book will prove invaluable both to postgraduate biomedical science students who are training to be cellular pathologists and to professionals working in diagnostic and research laboratories as part of their continuing professional development.
Most textbooks on neurodegenerative disorders have used a classification scheme based upon either clinical syndromes or anatomical distribution of the pathology. In contrast, this book looks to the future and uses a classification based upon molecular mechanisms, rather than clinical or anatomical boundaries. Major advances in molecular genetics and the application of biochemical and immunocytochemical techniques to neurodegenerative disorders have generated this new approach. Throughout most of the current volume, diseases are clustered according to the proteins that accumulate within cells (e.g. tau, α-synuclein and TDP-43) and in the extracellular compartments (e.g. β-amyloid and prion proteins) or according to a shared pathogenetic mechanism, such as trinucleotide repeats, that are a feature of specific genetic disorders. Chapters throughout the book conform to a standard lay-out for ease of access by the reader and are written by a panel of International Experts Since the first edition of this book, major advances have been made in the discovery of common molecular mechanisms between many neurodegenerative diseases most notably in the frontotemporal lobar degenerations (FTLD) and motor neuron disease or amyotrophic lateral sclerosis. This book will be essential reading for clinicians, neuropathologists and basic neuroscientists who require the firm up-to-date knowledge of mechanisms, diagnostic pathology and genetics of Neurodegenerative diseases that is required for progress in therapy and management.
Fish Pathology is the definitive, classic and essential book on the subject, providing in-depth coverage across all major aspects of fish pathology. This new, fully updated and expanded fourth edition builds upon the success of the previous editions which have made Fish Pathology the best known and most respected book in the field, worldwide.Commencing with a chapter covering the aquatic environment, the book provides comprehensive details of the anatomy and physiology of teleosts, pathophysiology and sytematic physiology, immunology, neoplasia, virology, parasitology, bacteriology, mycology, nutritional pathology and other non-infectious diseases. A final chapter provides extremely useful details of the most widely-used and trusted laboratory methods in the area. Much new infomation is included in this new edition, including enhanced coverage of any diseases which have become commercially significant since publication of the previous edition Beautifully illustrated in full colour throughout with many exceptional photographs, Fish Pathology, Fourth Edition, is an essential purchase for fish pathologists, fish veterinarians, biologists, microbiologists and immunologists, including all those working in diagnostic services worldwide. Personnel working in fish farming and fisheries will also find much of great use and interest within the book's covers. All libraries in universities and research establishments where biological and veterinary sciences are studied and taught should have copies of this landmark publication on their shelves.
A comprehensive look at empirical approaches to molecular discovery, their relationships with rational design, and the future of both Empirical methods of discovery, along with serendipitous and rational design approaches, have played an important role in human history. Searching for Molecular Solutions compares empirical discovery strategies for biologically useful molecules with serendipitous discovery and rational design, while also considering the strengths and limitations of empirical pathways to molecular discovery. Logically arranged, this text examines the different modes of molecular discovery, empha-sizing the historical and ongoing importance of empirical strategies. Along with a broad overview of the subject matter, Searching for Molecular Solutions explores: The differing modes of molecular discovery Biological precedents for evolutionary approaches Directed evolutionary methods and related areas Enzyme evolution and design Functional nucleic acid discovery Antibodies and other recognition molecules General aspects of molecular recognition Small molecule discovery approaches Rational molecular design The interplay between empirical and rational strategies and their ongoing roles in the future of molecular discovery Searching for Molecular Solutions covers several major areas of modern research, development, and practical applications of molecular sciences. This text offers empirical-rational principles of broad relevance to scientists, professionals, and students interested in general aspectsof molecular discovery, as well as the thought processes behind experimental approaches. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.
Equine Clinical Pathology is the first complete resource for hematology and clinical chemistry in horses. Encompassing the basic principles and advanced interpretation, the book’s single-species approach to pathology allows for focused coverage of the unique disease characteristics of equids. Equine Clinical Pathology is equally useful for anyone using clinical pathology as a diagnostic tool, from beginning student to experienced specialist. The heart of the book is organized by body system, making it easy to find and apply information. Chapters cover general laboratory medicine, including instruments and techniques, hematology, and proteins as well as specific organs, such as the kidney and liver. Equine Clinical Pathology is a useful bench-side reference for anyone involved in laboratory medicine for the horse.
In the current era of complete genome sequencing, Bioinformatics and Molecular Evolution provides an up-to-date and comprehensive introduction to bioinformatics in the context of evolutionary biology. This accessible text: provides a thorough examination of sequence analysis, biological databases, pattern recognition, and applications to genomics, microarrays, and proteomics emphasizes the theoretical and statistical methods used in bioinformatics programs in a way that is accessible to biological science students places bioinformatics in the context of evolutionary biology, including population genetics, molecular evolution, molecular phylogenetics, and their applications features end-of-chapter problems and self-tests to help students synthesize the materials and apply their understanding is accompanied by a dedicated website – www.blackwellpublishing.com/higgs – containing downloadable sequences, links to web resources, answers to self-test questions, and all artwork in downloadable format (artwork also available to instructors on CD-ROM). This important textbook will equip readers with a thorough understanding of the quantitative methods used in the analysis of molecular evolution, and will be essential reading for advanced undergraduates, graduates, and researchers in molecular biology, genetics, genomics, computational biology, and bioinformatics courses.
Molecular modeling encompasses applied theoretical approaches and computational techniques to model structures and properties of molecular compounds and materials in order to predict and / or interpret their properties. The modeling covered in this book ranges from methods for small chemical to large biological molecules and materials. With its comprehensive coverage of important research fields in molecular and materials science, this is a must-have for all organic, inorganic and biochemists as well as materials scientists interested in applied theoretical and computational chemistry. The 28 chapters, written by an international group of experienced theoretically oriented chemists, are grouped into four parts: Theory and Concepts; Applications in Homogeneous Catalysis; Applications in Pharmaceutical and Biological Chemistry; and Applications in Main Group, Organic and Organometallic Chemistry. The various chapters include concept papers, tutorials, and research reports.
This third in the Current Topics in Molecular Cell Biology and Molecular Medicine Series contains a careful selection of new and updated, high-quality articles from the well-known Meyer's Encyclopedia, describing new perspectives in stem cell research. The 26 chapters are divided into four sections: Basic Biology, Stem Cells and Disease, Stem Cell Therapy Approaches, and Laboratory Methods, with the authors chosen from among the leaders in their respective fields. This volume represents an essential guide for students and researchers seeking an overview of the field.
Molecular Markers in Plants surveys an array of technologies used in the molecular analysis of plants. The role molecular markers play in plant improvement has grown significantly as DNA sequencing and high-throughput technologies have matured. This timely review of technologies and techniques will provide readers with a useful resource on the latest molecular technologies. Molecular Markers in Plants not only reviews past achievements, but also catalogs recent advances and looks forward towards the future application of molecular technologies in plant improvement. Opening chapters look at the development of molecular technologies. Subsequent chapters look at a wide range of applications for the use of these advances in fields as diverse as plant breeding, production, biosecurity, and conservation. The final chapters look forward toward future developments in the field. Looking broadly at the field of molecular technologies, Molecular Markers in Plants will be an essential addition to the library of every researcher, institution, and company working in the field of plant improvement.
This advanced text introduces to the advanced undergraduate and graduate student the mathematical foundations of the methods needed to carry out practical applications in electronic molecular quantum mechanics, a necessary preliminary step before using commercial programmes to carry out quantum chemistry calculations. Major features of the book include: Consistent use of the system of atomic units, essential for simplifying all mathematical formulae Introductory use of density matrix techniques for interpreting properties of many-body systems An introduction to valence bond methods with an explanation of the origin of the chemical bond A unified presentation of basic elements of atomic and molecular interactions The book is intended for advanced undergraduate and first-year graduate students in chemical physics, theoretical and quantum chemistry. In addition, it is relevant to students from physics and from engineering sub-disciplines such as chemical engineering and materials sciences.
The classic and authoritative textbook, Molecular Mechanisms of Photosynthesis,is now fully revised and updated in this much-anticipated second edition. Whilst retaining the first edition’s clear writing style and accessible description of this complex process, updates now include cutting-edge applications of photosynthesis, such as to bioenergy and artificial photosynthesis as well as new analytical techniques. Written by a leading authority in photosynthesis research, this new edition is presented in full color with clear, student-friendly illustrations. An interdisciplinary approach to photosynthesis is taken, with coverage including the basic principles of energy storage, the history and early development of photosynthesis, electron transfer pathways, genetics and evolution. A comprehensive appendix, containing an introduction to the basic chemical and physical principles involved in photosynthesis, is also included. Molecular Mechanisms of Photosynthesis,second edition, is an indispensable text for all students of plant biology, bioenergy, and molecular biology, in addition to researchers in these and related fields looking for an accessible introduction to this vital and integral process to life on earth. stresses an interdisciplinary approach emphasizes recent advances in molecular structures and mechanisms includes the latest insights and research on structural information, improved techniques as well as advances in biochemical and genetic methods comprehensive appendix, which includes a detailed introduction to the physical basis of photosynthesis, including thermodynamics, kinetics, and spectroscopy associated website with downloadable figures as powerpoint slides for teaching
Now in full color throughout, Duncan and Prasse’s Veterinary Laboratory Medicine: Clinical Pathology, Fifth Edition offers a comprehensive overview of hematology, hemostasis, clinical chemistry, urinalysis, cytology, and reference intervals in a highly accessible outline format. With information on all major domestic species, the text is designed for the reader to quickly find answers to clinical questions. Taking a problem-solving approach to the interpretation of laboratory data, this book includes clinical cases to illustrate the concepts of laboratory data interpretation, with tables and key words to aid readers in locating and applying information. The fifth edition has been fully revised to reflect the latest knowledge, diagnostic methods, and practices in veterinary laboratory medicine. A companion website provides the images in PowerPoint and references linked to PubMed at www.wiley.com/go/latimer. Duncan and Prasse’s Veterinary Laboratory Medicine is an excellent quick reference for practicing veterinarians, veterinary students, clinical interns and residents, and pathology residents.
Clinical Pathology for the Veterinary Team is a concise, reliable resource for technical team members performing clinical diagnostic evaluation. Focusing on sample handling, machinery calibration, normal physiology and anatomy, clinical diagnostics, and disease processes, the book helps technical team members to consistently achieve high-quality results in small animal lab work. An essential bench-side reference for in-house laboratories, the book includes a DVD with interactive cases to build skills in reading and interpreting digital slides. With chapters on blood counts, chemistry, urinalysis and electrolytes, advanced diagnostics, and cytology, Clinical Pathology for the Veterinary Team offers key technical information for consistently obtaining and evaluating samples. The book is a practical resource for anyone performing lab work, including veterinary technicians, technician and veterinary students, and veterinarians.
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Written by a team of international experts, this book provides an authoritative overview and practical guide to the molecular biology and genetic basis of haematologic cancers including leukemia. Focusing on the importance of cytogenetics and related assays, both as diagnostic tools and as a basis for translational research, this is an invaluable guide for basic and clinical researchers with an interest in medical genetics and haemato-oncology. The Genetic Basis of Haematological Cancers reviews the etiology and significance of genetic and epigenetic defects that occur in malignancies of the haematopoietic system. Some of these chromosomal and molecular aberrations are well established and already embedded in clinical management, while many others have only recently come to light as a result of advances in genomic technology and functional investigation. The book includes seven chapters written by clinical and academic leaders in the field, organised according to haematological malignancy sub-type. Each chapter includes a background on disease pathology and the genetic abnormalities most commonly associated with the condition. Authors present in-depth discussions outlining the biological significance of these lesions in pathogenesis and progression, and their use in diagnosis and monitoring response to therapy. The current or potential role of specific abnormalities as novel therapeutic targets is also discussed. There is also a full colour section containing original FISH, microarrays and immunostaining images.