Progress in Nucleic Acid Research and Molecular Biology provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contributions from leaders in their fields and abundant references.* This series provides a forum for discussion of new discoveries, approaches, and ideas* Contributions from leading scholars and industry experts* Reference guide for researchers involved in molecular biology and related fields
With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include: chemical synthesis of modified RNA expansion of the genetic alphabet in nucleic acids by creating new base pairs chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides nucleic-acid-templated chemistry chemical biology of peptide nucleic acids (PNA) the interactions of small molecules with DNA and RNA the architectural modules of folded RNAs genesis and biological applications of locked nucleic acid (LNA) small non-coding RNA in bacteria microRNA-guided gene silencing nucleic acids based therapies innate immune recognition of nucleic acid light-responsive nucleic acids for the spatiotemporal control of biological processes DNA methylation frameworks for programming RNA devices RNA as a catalyst: The Diels-Alderase-Ribozyme evolving an understanding of RNA function by in vitro approaches the chemical biology of aptamers: synthesis and applications nucleic acids as detection tools bacterial riboswitch discovery and analysis The Chemical Biology of Nucleic Acids is an essential compendium of the synthesis of nucleic acids and their biological applications for bioorganic chemists, chemical biologists, medicinal chemists, cell biologists, and molecular biologists.
This book “MOLECULAR CHARACTERIZATION OF RIDGE GOURD (Luffa acutangula L.) AND SPONGE GOURD (Luffa cylindrica L.) GENOTYPES THROUGH PCR BASED MOLECULAR MARKERS” contain molecular Marker and biochemical marker application in ridge gourd and sponge gourd genotypes.Molecular biology is the ever-accelerating rate of progress, both in the development of new methodologies and in the practical applications of these methodologies. These deal primarily with core nucleic acid techniques and protein expression through biochemical and genetic detection methods. As such, this book should prove of interest both to undergraduates studying for biological or chemical qualifications and to postgraduate and other scientific workers who need a sound introduction to this ever rapidly advancing and expanding area. The genotype identification through molecular and biochemical markers resulted in developing moderate diversified dendrogram of 17 ridge gourd and sponge gourd genotypes.
Advances in molecular technologies over recent years have a profound impact on diagnostic pathology. Molecular biology techniques utilize DNA, RNA, and enzymes that interact with nucleic acids to understand biology at a molecular level. Information derived from genomics and proteomics have permitted improved insights into a range of disorders and have led to improvements in the understanding and classification of many diseases. These advances will provide a complementary approach to traditional methods of histopathological analysis in the future. Molecular analysis will significantly impact three major areas: specific diagnosis, prognosis determination and prediction of response to therapy. The pathologist will continue to play a fundamental, role in diagnosis and will likely be in a pivotal position to guide the implementation and interpretation of these tests as they move from the research laboratory into diagnostic pathology.
Cardiovascular diseases are life-threatening conditions which can develop through the misregulation of complex cellular signaling networks. Several surveillance mechanisms have evolved to discriminate self from foreign nucleic acids and accordingly trigger effective cellular responses to target the pathogenic threats. Several efforts have been made to understand the role of pattern recognition receptors (PRRs) and nucleic acids in this condition. Understanding these molecular interactions is not only important for exploring the molecular mechanisms underlying cardiac pathophysiology, but also in the development of novel therapeutics. The application of interdisciplinary approaches that integrate high-throughput genomics, proteomics, biostatistics, and computational biology will open avenues to novel multi-hit therapeutic approaches for cardiac diseases. Here, we describe the current knowledge in nucleic acids research in relation to their discrimination, immune activation, and signaling pathways and their potential to cause cardiac pathophysiology.
This monograph described the second project of the author, updated from the original Chinese vision “Functionalization and Reconstruction of Oligonucleotides”, completed in Peking University. A novel acyclic nucleic acid mimic based on (S)-3-Amino-1,2-propanediol was synthesized and evaluated in duplexes and quardruplexes, which showed superior biostability and would probably find its application as diagnose and therapy agents. This research would shed light on further research on artificial nucleic acids.
This third in the Current Topics in Molecular Cell Biology and Molecular Medicine Series contains a careful selection of new and updated, high-quality articles from the well-known Meyer's Encyclopedia, describing new perspectives in stem cell research. The 26 chapters are divided into four sections: Basic Biology, Stem Cells and Disease, Stem Cell Therapy Approaches, and Laboratory Methods, with the authors chosen from among the leaders in their respective fields. This volume represents an essential guide for students and researchers seeking an overview of the field.
This book represents the most comprehensive and up-to-date collection of information on the topic of computational molecular biology. Bringing the most recent research into the forefront of discussion, Algorithms in Computational Molecular Biology studies the most important and useful algorithms currently being used in the field, and provides related problems. It also succeeds where other titles have failed, in offering a wide range of information from the introductory fundamentals right up to the latest, most advanced levels of study.
A number of molecular biology manuals are commercially available that cover various molecular biology protocols but these manuals are far away from the approach of the students due to their high cost and difficult scientific language. The purpose of this manual is to provide the authentic material to the students in affordable price. A very simple language is used in the manual so it is easy to understand. This manual covers the basic principle, reagents, equipment and protocols for various experiments/techniques that are commonly used in the molecular biology research. The manual is an easy way for practical understanding of different aspects of molecular biology. I would like to thank the contributing authors for sparing their time and writing different chapters for this book.
Recent advances in genetic research have produced a surge of student interest in molecular biology/ molecular genetics courses. Molecular biology is a fast-paced field that constantly provides new insights into the living world and the role of humans within it. From a wide area of molecular biology, molecular genetics represents one of the major technological field of the twenty-first century. Its wide ranging, multi-disciplinary activities include polymerase cahin reactions, molecular marker based studies and the gene cloning. One of the main challenges for the instructor in such a course is to lacking of the proper and manageable practical handling during lab experimentation's.
In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
DESCRIPTION:International Review of Cell & Molecular Biology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research Key Features* Authored by some of the foremost scientists in the field* Provides up-to-date information and directions for future research* Valuable reference material for advanced undergraduates, graduate students and professional scientists
The family Solanaceae is unique in representing crops like potato, tomato, eggplant, and pepper that are of importance to both arable and horticultural sectors. Its economic importance, and recent progress in molecular biology, has propelled the Solanaceae to the forefront of scientific importance in the fields of comparative genomics and plastome biology research. Tomato (Solanum lycopersicum L.) cv. MicroTom is a miniature-dwarf-determinate. It differs from standard tomato cultivars by only two recessive genes which provide the dwarf phenotype to MicroTom. Like the model plant species Arabidopsis thaliana, short life cycle, well-characterized genetics, and small DNA content per haploid genome, have recently led to its adoption as model research material for the Solanaceae. Efficient methods for in vitro propagation, regeneration, and transformation of plants are of pivotal importance to both basic and applied research.
The book consists of ten chapters that present the results of the research work on ionic effect on nucleic acid. The structure and stability of several non-natural triplets are also analysed and comparison has been made with that of natural triplets of nucleic acid. Each chapter explains the objective of the work, and focuses on results and conclusion based on the findings. The thorough explanations of the ab initio method used in the study are included. Both quantum mechanics and molecular mechanics calculations used in the work are illustrated in this chapter.