With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. Topics covered include: chemical synthesis of modified RNA expansion of the genetic alphabet in nucleic acids by creating new base pairs chemical biology of DNA replication: probing DNA polymerase selectivity mechanisms with modified nucleotides nucleic-acid-templated chemistry chemical biology of peptide nucleic acids (PNA) the interactions of small molecules with DNA and RNA the architectural modules of folded RNAs genesis and biological applications of locked nucleic acid (LNA) small non-coding RNA in bacteria microRNA-guided gene silencing nucleic acids based therapies innate immune recognition of nucleic acid light-responsive nucleic acids for the spatiotemporal control of biological processes DNA methylation frameworks for programming RNA devices RNA as a catalyst: The Diels-Alderase-Ribozyme evolving an understanding of RNA function by in vitro approaches the chemical biology of aptamers: synthesis and applications nucleic acids as detection tools bacterial riboswitch discovery and analysis The Chemical Biology of Nucleic Acids is an essential compendium of the synthesis of nucleic acids and their biological applications for bioorganic chemists, chemical biologists, medicinal chemists, cell biologists, and molecular biologists.
Molecular biology for advanced learners - was designed for under graduate and post graduate medical, paramedical, nursing, pharmacy and other life sciences students. This book was mainly focused in explaining "central dogma of molecular biology" to students in simple language. Mile stones in the molecular biology were given point to point in the beginning of the book. First chapter starts with nucleic acid chemistry, followed by metabolism of purines and pyrimidines. In the next chapters' simple and brief explanation of replication, transcription and translation were given. Recent technology in molecular biology like gene therapy, gene mapping, gene expression and PCR were given in the later chapters.
The book consists of ten chapters that present the results of the research work on ionic effect on nucleic acid. The structure and stability of several non-natural triplets are also analysed and comparison has been made with that of natural triplets of nucleic acid. Each chapter explains the objective of the work, and focuses on results and conclusion based on the findings. The thorough explanations of the ab initio method used in the study are included. Both quantum mechanics and molecular mechanics calculations used in the work are illustrated in this chapter.
Advances in molecular technologies over recent years have a profound impact on diagnostic pathology. Molecular biology techniques utilize DNA, RNA, and enzymes that interact with nucleic acids to understand biology at a molecular level. Information derived from genomics and proteomics have permitted improved insights into a range of disorders and have led to improvements in the understanding and classification of many diseases. These advances will provide a complementary approach to traditional methods of histopathological analysis in the future. Molecular analysis will significantly impact three major areas: specific diagnosis, prognosis determination and prediction of response to therapy. The pathologist will continue to play a fundamental, role in diagnosis and will likely be in a pivotal position to guide the implementation and interpretation of these tests as they move from the research laboratory into diagnostic pathology.
Cardiovascular diseases are life-threatening conditions which can develop through the misregulation of complex cellular signaling networks. Several surveillance mechanisms have evolved to discriminate self from foreign nucleic acids and accordingly trigger effective cellular responses to target the pathogenic threats. Several efforts have been made to understand the role of pattern recognition receptors (PRRs) and nucleic acids in this condition. Understanding these molecular interactions is not only important for exploring the molecular mechanisms underlying cardiac pathophysiology, but also in the development of novel therapeutics. The application of interdisciplinary approaches that integrate high-throughput genomics, proteomics, biostatistics, and computational biology will open avenues to novel multi-hit therapeutic approaches for cardiac diseases. Here, we describe the current knowledge in nucleic acids research in relation to their discrimination, immune activation, and signaling pathways and their potential to cause cardiac pathophysiology.
This monograph described the second project of the author, updated from the original Chinese vision “Functionalization and Reconstruction of Oligonucleotides”, completed in Peking University. A novel acyclic nucleic acid mimic based on (S)-3-Amino-1,2-propanediol was synthesized and evaluated in duplexes and quardruplexes, which showed superior biostability and would probably find its application as diagnose and therapy agents. This research would shed light on further research on artificial nucleic acids.
This book represents the most comprehensive and up-to-date collection of information on the topic of computational molecular biology. Bringing the most recent research into the forefront of discussion, Algorithms in Computational Molecular Biology studies the most important and useful algorithms currently being used in the field, and provides related problems. It also succeeds where other titles have failed, in offering a wide range of information from the introductory fundamentals right up to the latest, most advanced levels of study.
This third in the Current Topics in Molecular Cell Biology and Molecular Medicine Series contains a careful selection of new and updated, high-quality articles from the well-known Meyer's Encyclopedia, describing new perspectives in stem cell research. The 26 chapters are divided into four sections: Basic Biology, Stem Cells and Disease, Stem Cell Therapy Approaches, and Laboratory Methods, with the authors chosen from among the leaders in their respective fields. This volume represents an essential guide for students and researchers seeking an overview of the field.
A number of molecular biology manuals are commercially available that cover various molecular biology protocols but these manuals are far away from the approach of the students due to their high cost and difficult scientific language. The purpose of this manual is to provide the authentic material to the students in affordable price. A very simple language is used in the manual so it is easy to understand. This manual covers the basic principle, reagents, equipment and protocols for various experiments/techniques that are commonly used in the molecular biology research. The manual is an easy way for practical understanding of different aspects of molecular biology. I would like to thank the contributing authors for sparing their time and writing different chapters for this book.
Recent advances in genetic research have produced a surge of student interest in molecular biology/ molecular genetics courses. Molecular biology is a fast-paced field that constantly provides new insights into the living world and the role of humans within it. From a wide area of molecular biology, molecular genetics represents one of the major technological field of the twenty-first century. Its wide ranging, multi-disciplinary activities include polymerase cahin reactions, molecular marker based studies and the gene cloning. One of the main challenges for the instructor in such a course is to lacking of the proper and manageable practical handling during lab experimentation's.
The family Solanaceae is unique in representing crops like potato, tomato, eggplant, and pepper that are of importance to both arable and horticultural sectors. Its economic importance, and recent progress in molecular biology, has propelled the Solanaceae to the forefront of scientific importance in the fields of comparative genomics and plastome biology research. Tomato (Solanum lycopersicum L.) cv. MicroTom is a miniature-dwarf-determinate. It differs from standard tomato cultivars by only two recessive genes which provide the dwarf phenotype to MicroTom. Like the model plant species Arabidopsis thaliana, short life cycle, well-characterized genetics, and small DNA content per haploid genome, have recently led to its adoption as model research material for the Solanaceae. Efficient methods for in vitro propagation, regeneration, and transformation of plants are of pivotal importance to both basic and applied research.
Finally, a stand-alone, all-inclusive textbook on yeast biology. Based on the feedback resulting from his highly successful monograph, Horst Feldmann has totally rewritten he contents to produce a comprehensive, student-friendly textbook on the topic. The scope has been widened, with almost double the content so as to include all aspects of yeast biology, from genetics via cell biology right up to biotechnology applications. The cell and molecular biology sections have been vastly expanded, while information on other yeast species has been added, with contributions from additional authors. Naturally, the illustrations are in full color throughout, and the book is backed by a complimentary website. The resulting textbook caters to the needs of an increasing number of students in biomedical research, cell and molecular biology, microbiology and biotechnology who end up using yeast as an important tool or model organism.
These two volumes contain a selection of updated articles from the acclaimed Meyers Encyclopedia of Molecular Cell Biology and Molecular Medicine, the most authoritative resource in cell and molecular biology, combined with new articles by «founding fathers» in the field. The work is divided into six sections: + Biological Basis + Modeling + Modular Parts and Circuits + Synthetic Genomes + Diseases and Therapeutics + Chemicals Production. Ideally suited as advanced reading for students and postdocs, and with all current research trends covered by an impressive number of leading figures in the field, this is the first choice reference for research institutions.
A comprehensive look at empirical approaches to molecular discovery, their relationships with rational design, and the future of both Empirical methods of discovery, along with serendipitous and rational design approaches, have played an important role in human history. Searching for Molecular Solutions compares empirical discovery strategies for biologically useful molecules with serendipitous discovery and rational design, while also considering the strengths and limitations of empirical pathways to molecular discovery. Logically arranged, this text examines the different modes of molecular discovery, empha-sizing the historical and ongoing importance of empirical strategies. Along with a broad overview of the subject matter, Searching for Molecular Solutions explores: The differing modes of molecular discovery Biological precedents for evolutionary approaches Directed evolutionary methods and related areas Enzyme evolution and design Functional nucleic acid discovery Antibodies and other recognition molecules General aspects of molecular recognition Small molecule discovery approaches Rational molecular design The interplay between empirical and rational strategies and their ongoing roles in the future of molecular discovery Searching for Molecular Solutions covers several major areas of modern research, development, and practical applications of molecular sciences. This text offers empirical-rational principles of broad relevance to scientists, professionals, and students interested in general aspectsof molecular discovery, as well as the thought processes behind experimental approaches. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.
Reproductive biology and Genetic diversity of Terminalia paniculata is designed to serve as a book for researchers in Biotechnology, Botany, Genetics and molecular biology. The book focuses on explaining the both basic and advanced concepts and techniques in reproductive biology, pollination, molecular biology, and genetic diversity and their application thereof. The book provide comprehensive discussions on pollination in tropical trees, nature of pollinators, nectar secretion pattern, breeding system, allozyme diversity and gene flow aspects.